Sample collection

The quality of laboratory test results depends on the proper collection and handling of the specimen. Correct patient preparation, specimen collection, labelling, packaging and transportation are essential factors in obtaining timely and valid test results.

Tube guide and recommended order of draw:

Paediatric tube guide:

See Pathology test A-Z for test specific collection information.

---

Particular note should be taken of:

• The need for prior notification of request to laboratory.
• Specimen integrity; e.g. special transport (including urgent transportation) and packaging requirements.
• Sample size requirements. When only a small amount of sample is obtained, indicate which test(s) are clinically most important.
• Use of preservatives/transport media.
• Time critical specimens (e.g. antibiotic assay, U & E, cortisol).
• Correct tubes/appropriate containers i.e. ONLY those provided/approved by laboratory.
• Correct sample(s) for request/test(s).
• “Order of Draw” chart for BD Vacutainer System to avoid sample contamination.
• Correct mixing of the sample post collection
• Delivery to correct laboratory in a timely fashion.

Samples should be sent to the laboratory with minimal delay. Samples received following an excessive period of time may be rejected.

Rejection of samples

• Specimens sent in an unsafe manner that pose handling and health hazards may be rejected.
• Specimens received that are not appropriate for the investigation requested will be rejected.
• Requests for inappropriate investigations will be rejected (e.g. Hepatitis C virus quantification without prior positive diagnostic serology).
• Request forms received without a sample will be rejected.
• Samples received without sufficient patient identifiers will be rejected (see sample labelling)

Requester will be notified verbally or via the reporting process in accordance with local procedures.

Guide to centrifuging samples (outside of Pathology laboratory)